androstenedione (a4) – serum Test

Androstenedione (A4) – Serum

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clinical definition of androstenedione (a4) – serum

Androstenedione is a C-19 (19 carbon atoms) steroid hormone found in men as well as in premenopausal women. Androstenedione is a steroid sex hormone which originates in the gonads, with minor contribution from the adrenal glands (1.5-3 mg/day); in postmenopausal women, the adrenal gland constitutes the major source of this hormone. This test is used when steroid abuse is suspected and also when women are suspected of virilizing syndromes i.e. a condition where females develop male sexual characteristics due to of high testosterone levels in them.

when do you expect results?

24 to 36 Hours

why get tested?

The test helps diagnose the causes of a high testosterone (male sex harmone) in women, routine health screenings and suspected disease or toxicity.To see the progress of a medication or treatment plan.Suspected cases of virilizing syndromes in womenSuspected anabolic steroid abuseMay be used along with 17-OH progesterone to diagnose CAH Management of CAH.

reason to take androstenedione (a4) – serum test


Common diagnosis and symptoms that are associated with androstenedione are early puberty, virilizing syndrome in females and relapse of menstural cycle in post-menopausal women.



preparations needed for androstenedione (a4) – serum test

Preparatory instructions during specimen collection*:No Fasting Required.No other special preparations required.

sample required?

Specimen type:Serum (Blood Sample) Specimen collection procedure:Venipuncture - Collection of blood from a vein, usually from the arm.

Understanding results ofAndrostenedione (A4) – Serum

Reference RangeInterpretation
Female 1 to 10 years8-50 ng/dL

‘*A Reference range is a set of values which helps the healthcare professional to interpret a medical test. It may vary with age, gender, and other factors. Reference ranges may also vary between labs, in value & units depending on instruments used and method of establishment of reference ranges’